Abstract: Shellac-wax emulsion was prepared by inverse phase method. Through single factor experiment to choose factors and levels, volume average particle diameter of the emulsion was optimized through response surface methodology with three factors and three levels based on principle of Box-Behnken design. The results showed that the optimum conditions were as follows: emulsifier dosage 5 g, rate of mixing 500 r/min, emulsifying temperature 88℃. Under the optimal conditions, the optimum volume-average particle size was 0.39 um. The particle size and distribution of the optimized shellac-wax emulsion were studied with laser granularity analyzer. The stability of the shellac-wax emulsion was tested by freezing-thawing experiment. The particle diameter of shellac-wax emulsion is well-distributed below 0.76 μm, and the emulsion possesses good freezing-thawing stability.