Abstract: The research advances in the main methods for biofuel production including enzymatic catalysis, catalytic cracking and catalytic hydroprocessing are introduced. It is pointed out that enzymatic catalysis is an environment-friendly method to produce bio-diesel with great industry prospect. Compared with enzymatic catalysis, catalytic cracking and catalytic hydroprocessing process generate fuels that have physical and chemical properties comparable to those specified for petroleum-based fuels. At last the existing main problem were mentioned and prospect of this research field is pointed out.