Abstract: The static adsorption and desorption effects of ten kinds of macroporous resins on B. striata polyphenols crude extract were compared, and the optimal process of purifying with LSA-21 resin was discussed. Furthermore, the antioxidant activities of the pre- and post-purification B. striata polyphenols were tested in vitro. The results showed that, at room temperature, the optimal process parameters for adsorption of crude extract with LSA-21 resin were as follows:the mass concentration of polyphenol crude extract 0.4-0.6 g/L, pH vlaue 4, and the loading velocity 2.0 mL/min. The optimal process parameters for desorption were as follows:desorption solution ethanol volume fraction 80%, pH vlaue 6, and elution flow rate 1.0 mL/min. The purity increased from 4.48% to 27.61% after purifying 100 mL of 0.47g/L crude extract under the optimal technological conditions, and the purification effect was good. The DPPH·scavenging activity of the purified B. striata polyphenols was obviously improved. The IC₅₀ of DPPH·、OH· and O₂^-·free radicals were 7.72, 8.65 and 205.39 mg/L respectively.