Immobilization of β-glucosidase on Chitosan Beads

β-Glucosidase was immobilized on chitosan beads by adsorption and crosslinking. The method of orthogonal test was used to investigate the optimal condition of immobilization. The optimal condition of immobilization was as follows: β-glucosidase dosage 48.6 IU/g dry chitosan was absorbed with chitosan beads at pH value 5.0 for 12 h, followed by crosslinking at 25℃ for 2 h in 1.0% glutaraldehyde(GA) solution. The activity of immobilized β-glucosidase and recovery rate of β-glucosidase activity were 41.76 IU/g (based on dry chitosan) and 85.93%, respectively.