STUDY ON RELATIONSHIP BETWEEN MOLECULAR WEIGHT OF SODIUM SULFATE-CELLULOSE AND ITS ANTICOAGULATION ACTIVITY

Sodium sulfate-cellulose (Na-CS) had high anticoagulation activity. In order to study the relationship between the molecular weights ( Mw) and the anticoagulation activity of Na-CS, it was essential to prepare a series of Na-CS analogues having different Mw with the same sulfate substitution degree. In this study, Na-CS samples having different Mw were obtained by combinated separation method of acid hydrolysis and gel filtration-ion exchange. With these samples as objects of study, the effects of Mw on the anticoagulation efficacy of Na-CS and the inhibition of coagulation factors Ⅱa and Xa were studied. It was indicated that, with the increasing of Mw of Na-CS in a certain range, the prolongation on activated partial thrombo-plastin time (APTT) and thrombin time (TT) was increased and the inhibition on IIa and Xa was improved.