EFFECTS OF MOLECULAR WEIGHT DISTRIBUTION OF XYLAN ON XYLANASE SYNTHESIS BY TRICHODERMA REESEI RUT C-30

Effects of molecular weight distribution of xylan on xylanase synthesis by Trichoderma reesei Rut C-30 have been investigated. By Sephadex G-100 column chromatography, it was found that xylan A contained more low- molecular weight components, and xylan B contained less low-molecular weight components, while xylan C contained the least low-molecular weight components. These xylans were used as carbon source for xylanase synthesis,and presented the highest xylanase activity at 153.64, 120.84, 110.84 IU/mL,and culture time were 60, 72, 96 h, respectively. When xylan A was used as the carbon source for xylanase synthesis, xylo-oligosacchrides were 80.70% of the total sugars in the hydrolysate, which were increased by 17.71% and 20.59%,compared to that xylanases synthesized with xylans B and C. It indicated that the more the low-molecular weight components in xylan, the better the xylanases were induced, and the less the culture time was required to obtain high xylanase activity, and low- molecular weight components of xylan were favorable to inducing endo-1,4-β-D-xylanase.