SEPARATION,PURIFICATION AND STRUCTURAL STUDIES OF POLYSACCHARIDES FROM POLYGONATUM KINGIANUM

Three polysaccharide components were isolated from Polygonatum kingianum Coll.et Hemsl, by hot water extraction,ethanol precipitation,and purification through DEAE-cellulose and gel permeation column chromatography. The structural feature of P. kingianum polysaccharide I (PKP I) was identified by chemical (including sugar composition analysis, methylation analysis and periodate oxidation) and spectral analyses (IR, 1H NMR and GC-MS). The results showed that PKP I was mainly composed of glucose, the main chain of polysaccharide was α -(1→ 4) linked, and mean molecular weight was 8100.