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元宝枫、三角枫及金沙枫叶片柯里拉京含量的变异与季节变化

Variation and Seasonal Difference of Corilagin Content in Leaves of Acer truncatum, Acer buergerianum and Acer paxii

    摘要
  • 摘要:
    目的 为进一步开发元宝枫、三角枫和金沙枫的药用价值，丰富用于提取柯里拉京的植物资源并提升这 3 种枫树的药用价值以及筛选药赏兼用型品种。
    方法 采用超高效液相色谱法（UPLC）对元宝枫、三角枫和金沙枫叶片中的柯里拉京进行测定，以XBridge C18 （4.6 × 150 mm, 3.5 µm）为色谱柱，以0.4%甲酸水溶液和甲醇为流动相，流速为0.3 mL·min−1，柱温为25 ℃，检测波长 270 nm，进样量2 μL。
    结果 元宝枫、三角枫和金沙枫的叶片中都含有柯里拉京；不同树种之间柯里拉京含量存在较大差异，三角枫含量最高，金沙枫次之，元宝枫最低；不同品种之间柯里拉京含量也存在较大差异，三角枫4个品种中‘兴旺’平均含量最高，而‘千里红’在10月达到6.888 7 mg·g−1的峰值；金沙枫‘鲁绿’在6月达最高值2.664 0 mg·g−1；元宝枫4个品种中，‘绿宝石’在6月达最高值0.607 9 mg·g−1，不同采收时间柯里拉京含量也存在较大差异，三角枫峰值多在10月，元宝枫和金沙枫最高值多出现在5月和6月。
    结论 首次揭示元宝枫、三角枫和金沙枫叶片中含有柯里拉京，其中，三角枫更适合作为提取柯里拉京的原材料，金沙枫和元宝枫可作为候选资源。

     

    Abstract:
    Objective To determine the content of corilagin in leaves of Acer truncatum Bunge, A. buergerianum Miq. and A. paxii Franch.
    MethodsThe ultra-performance liquid chromatography (UPLC) were used. XBridge C18 (4.6 × 150 mm, 3.5 µm) was used as the chromatographic column, and 0.4% formic acid aqueous solution and methanol as the mobile phase with the flow rate of 0.3 mL·min−1, the column temperature of 25 ℃, the detection wavelength of 270 nm, and the injection volume of 2 μL.
    ResultsThe leaves of A. truncatum Bunge, A. buergerianum Miq. and A. paxii Franch. contained corilagin. There were significant differences in the content of corilagin among different tree species. The content in A. buergerianum Miq. was the highest, followed by A. paxii Franch. and A. truncatum Bunge. There were also significant differences in the content of corilagin among different varities. Among the four varieties of A. truncatum Bunge, ‘Lvbaoshi’ reached the highest value of 0.607 9 mg·g−1, the average content in ‘Xingwang’ among the four cultivars of A. buergerianum Miq. was the highest, and ‘Qianlihong’ had the highest value (6.888 7 mg·g−1) in October, and the highest valuein A. paxii Franch. ‘Lulv’ was 2.664 0 mg·g−1. In addition, there was also a big difference in the content of corilagin at different harvest times. The highest values of A. truncatum Bunge and A. paxii Franch. mostly appeared in May and June, and the best harvesting time of A. buergerianum Miq. was in October.
    Conclusion This is the first time for revealing that there is corilagin in the leaves of A. truncatum Bunge, A. buergerianum Miq. and A. paxii Franch., among which A. buergerianum Miq. is more suitable as the raw material for extracting corilagin, and A. truncatum Bunge and A. paxii Franch. can be used as candidate resources.

     

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