Objective To identify AMT gene family members in Betula platyphylla and analyze the expression pattern of AMT genes.

Method Bioinformatics methods were used to identify the family genes and real-time fluorescence quantitative analysis of gene expression.

Result 9 AMT genes were identified from the B.platyphylla genome and divided into two subfamilies, AMT1 and AMT2, named BpAMT1.1-1.4 and BpAMT2.1-2.5; These BpAMT proteins contained 384-522 amino acid residues with isoelectric points ranging from 4.61 to 8.16, and all of which were located on the plasma membrane and organelle membrane; The genes of BpAMT family were distributed on 5 chromosomes unevenly, and there were tandem duplication among the members. BpAMTs’ expression patterns had specificity in different tissues, showing a leaf>root>stem trend; At the same time, KNO₃, NH₄Cl, MeJA, GA₃, ABA, CdCl₂ and diurnal variation could affect the expression of BpAMT gene, and the response of members differed under different treatments.

Conclusion 9 BpAMT genes are identified and divided into two subfamilies. BpAMT gene plays an important role in regulating nitrogen uptake, transport and in response to hormone signals or abiotic stress. These results lay a foundation for further analysis of the function of BpAMT gene in growth and resistance to stress in B.platyphylla.