Objective The study aimed to analyze the genomic basic information of the biocontrol Trichoderma songyi M75 strain, which has strong antagonistic activity against the pine shoot blight pathogen Spaeropsis sapinea.

Method The third-generation sequencing technology based on Illumina NovaSeq PE 150 was used to sequence the genome of T. songyi M75 strain.

Result The total genome length of T. songyi M75 was 34 483 860 bp, and the GC content was 49.50%, which was consistent with the characteristics of Trichoderma spp. In GO database, a total of 28 494 genes were annotated. In the KEGG database, 8 192 genes were enriched in 383 different tertiary metabolic pathways in KEGG, mainly concentrated in metabolic pathways (848), biosynthesis of secondary metabolites (330), antibiotic biosynthesis(243), microbial metabolism in different environments(240), amino acid biosynthesis(118), carbon metabolism(112) and other metabolic pathways. A total of 2 184 genes were annotated with protein functions in the KOG database. In the CAZy database of carbohydrate active enzymes, there were 259 glycoside hydrolase GHs genes, 105 glycosyltransferase GTs genes, 7 polysaccharide lyase PLs genes, 21 carbohydrate lipase CEs genes, and 54 carbohydrate binding component CBMs genes. The whole genome of M75 contains 41 gene clusters and 451 genes, most of which are related to the synthesis of secondary metabolites.

Conclusion This study obtained genomic sequence information of the biocontrol fungus T. songyi for the first time providing data support and important references for the genetic information, antibacterial mechanisms, and metabolic pathways of antibacterial substances in Trichoderma spp.