Abstract:
ObjeciveTo analyze the composition of volatile organic compounds produced by the biocontrol strain Trichoderma songyi M75.
Method Using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPMEGC-MS), volatile organic components of T. songyi M75 were analyzed and the antibacterial activity of some of the substance components was tested.
Result There were 18 volatile components successfully identified from M75 strain, including aldehydes, terpenes, ketones, alkanes, aromatics and esters. The content of 6-amyl-2H-pyran-one was the highest, with a relative content of 18.56%, but linalool had the best inhibitory effect against S. sapinea, with a relative content of 5.86%. Linalool had a wide antibacterial spectrum and also had good inhibitory effects on a variety of plant pathogens such as Cytospora chrysosperma, Cryphonectria parastica and Alternria sp, with an inhibition rate of more than 70.00%.
Conclusion Trichoderma spp generally have the ability to produce volatile organic compounds. This study successfully analyzed the volatile organic compounds produced by Trichoderma songyi M75 strain, providing scientific basis for the antibacterial mechanism of Trichoderma spp and the metabolism of antibacterial substances, and laying the foundation for the development of biocontrol agents using Trichoderma spp.
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