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湿地松纸浆材性状遗传变异分析及家系选择

Genetic Variation Analysis and Family Selection for Pulpwood Traits in Slash pine (Pinus elliottii)

  • 摘要:
    目的 解析湿地松纸浆材相关性状的遗传变异规律,筛选速生、优质长纤维纸浆材家系。
    方法 以33个完全随机区组设计(RCBD)的湿地松半同胞家系试验林为材料,30 a树龄时测定其胸径、基本密度、木材纤维形态(包括纤维长度、纤维宽度、卷曲指数、扭结指数等7个性状)和化学特性(包括木质素含量、纤维素含量和半纤维素含量共3个性状)等12个性状,基于混合线性模型(MLM)和最佳无偏预测(BLUP)分析遗传变异并估算遗传参数和育种值,利用综合选择指数法筛选速生、优质长纤维纸浆材家系。
    结果 方差分析显示,除了纽结角和纽结指数外的10个性状在湿地松半同胞家系间均有显著差异。纤维长度、宽度和纤维素含量等纤维性状的家系遗传力均较高,在0.50以上;当入选率达5%时纤维素含量的遗传增益达28%,而纤维素含量与胸径仅有弱遗传相关(|rg|<0.05),因此纸浆材选择主要考虑生长与纤维含量及其质量相关的6个性状:胸径、密度、纤维长、纤维素长宽比、纤维素含量和木质素含量。结合各家系育种值、选择指数等指标,筛选出4个湿地松优良家系,入选率为12.12%,入选家系相关性状的遗传增益平均值为2.18%~10.18%。
    结论 湿地松半同胞家系间生长和纤维相关性状差异显著,纤维素含量及纤维长宽等性状均具有较高的遗传力,利用指数选择法筛选了4个速生、纤维含量高的优质纸浆材家系,可用于湿地松纸浆材品种选育。

     

    Abstract:
    Objective Slash pine (Pinus elliottii) is an important conifer species for pulpwood production in China. In recent years, with the increasing demand for pulpwood in the country, genetic improvement of pulpwood traits and the selection of superior families of slash pine have become research priorities. Fiber morphology and chemical composition of wood directly affect pulp quality and papermaking performance. This study aimed to analyze the genetic variation patterns of fiber and chemical traits in half-sibling families of slash pine, providing a scientific basis for wood property improvement and the breeding of new superior varieties.
    Methods A total of 33 half-sibling families of 30-year-old P. elliottii from the Changle Forest Farm in Zhejiang Province were evaluated. Twelve traits were measured, including diameter at breast height (DBH), basic density, fiber morphology (e.g., fiber length, fiber width, curl index, kink index), and chemical composition (lignin, cellulose, and hemicellulose contents). Genetic variation analysis, correlation analysis, principal component analysis, and superior family selection were conducted.
    Results Significant differences were observed among families in DBH, basic density, fiber morphology, and chemical composition. Traits such as fiber length, fiber length-to-width ratio, and chemical composition exhibited strong genetic control. Positive genetic correlations were observed between DBH and fiber width as well as fiber length-to-width ratio, whereas negative correlations were found with cellulose and lignin contents. Based on breeding values, selection indices, and industrial requirements, four superior families were selected, with a selection rate of 12.12%. The selected families showed average genetic gains of 10.18% in DBH, 2.18% in basic density, 5.75% in fiber length, 5.67% in fiber length-to-width ratio, and 2.47% in cellulose content.
    Conclusion The selected families exhibited significant genetic gains in pulpwood traits, meeting the industrial requirements of the industry for high-quality papermaking. These results provide theoretical and practical guidance for future genetic improvement and germplasm innovation of P. elliottii.

     

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