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文冠果AP2/ERF基因家族的全基因组鉴定及表达分析

Genome-wide Identification and Expression Analysis of the AP2/ERF Gene Family in Xanthoceras sorbifolium Bunge

    摘要
  • 摘要:
    目的 对文冠果中AP2/ERF（APETALA2/乙烯反应元件结合因子）基因家族的染色体定位、基因结构、启动子中顺式作用元件以及盐雾胁迫下的表达模式进行分析，为挖掘XsAP2/ERF在盐雾胁迫下的作用提供参考。
    方法 基于文冠果基因组数据，利用NCBI数据库、MEGA7.0等生物信息学工具对文冠果AP2/ERF基因家族进行鉴定分析。以盐雾（饱和氯化钠）胁迫0、2和4 d的文冠果幼苗叶片为材料，进行转录组测序，分析文冠果AP2/ERF基因的表达规律。
    结果 （1）在文冠果全基因组中鉴定出145个AP2/ERF基因，这些基因在15条染色体上不均匀分布。（2）通过系统进化和结构域分析，将文冠果AP2/ERF基因家族分为AP2（21个成员）、ERF（119个成员）和RAV（5个成员）3个亚家族，共鉴定出10个保守基序，仅有motif 1 为该基因家族的共有基序；且在文冠果AP2/ERF基因家族中有90个基因不含内含子。（3）在文冠果AP2/ERF基因启动子区域共发现19种，共1 930个顺式作用元件，涵盖非生物胁迫、生长发育、植物激素响应三大类，其中，ABA应答元件数量最多（432个）；并通过互作分析，筛选出4个与文冠果AP2/ERF转录因子置信度较高的互作蛋白。（4）筛选到10个文冠果AP2/ERF基因（XsAP2/ERF2、XsAP2/ERF22、XsAP2/ERF23、XsAP2/ERF27、XsAP2/ERF48、XsAP2/ERF56、XsAP2/ERF103、XsAP2/ERF111、XsAP2/ERF122、XsAP2/ERF128）在盐雾胁迫期间持续显著上调表达。
    结论 XsAP2/ERF家族基因在文冠果响应盐雾胁迫过程中有着重要作用，本项研究对于今后XsAP2/ERF家族基因生物学功能的鉴定与抗盐基因资源的挖掘具有推动作用。

     

    Abstract:
    Objective This study aimed to analyze the chromosomal localization of the AP2/ERF（APETALA2/Ethylene - responsive Element Binding Factor） gene family in Xanthoceras sorbifolium Bunge, the gene structure, the cis-acting elements in the promoter, and the expression patterns under salt mist stress, providing a reference for exploring the role of XsAP2/ERF under salt mist stress.
    Methods AP2/ERF gene family members were systematically identified using bioinformatics tools (NCBI database, GSDS2.0, MEGA7.0) based on the X. sorbifolium genome. Transcriptome sequencing was performed on leaves of X. sorbifolium seedlings subjected to 0-, 2-, and 4-day salt spray stress (saturated NaCl solution) to investigate AP2/ERF gene expression dynamics.
    Results (1) A total of 145 AP2/ERF genes were identified in the whole genome of X. sorbifolium, unevenly distributed on 15 chromosomes.(2) Through phylogenetic and domain analysis, the AP2/ERF gene family of X. sorbifolium was divided into three subfamilies: AP2 (21 genes), ERF (119 genes), and RAV (5 genes). A total of 10 conserved motifs were identified, and only motif 1 was the common motif of this gene family. Moreover, 90 genes contained no introns.(3) A total of 19 types and 1930 cis-acting elements were found in the promoter region of AP2/ERF genes of X. sorbifolium, related to abiotic stress, growth and development, and phytohormone response. Among them, ABA-responsive elements were the most abundant (432). Interaction network analysis revealed four proteins with strong predicted interactions with AP2/ERF transcription factors.(4) Ten AP2/ERF genes of X. sorbifolium (XsAP2/ERF2, XsAP2/ERF22, XsAP2/ERF23, XsAP2/ERF27, XsAP2/ERF48, XsAP2/ERF103, XsAP2/ERF111, XsAP2/ERF122, XsAP2/ERF128) were significantly and continuously upregulated during salt mist stress.
    Conclusion The XsAP2/ERF gene family plays an important role in the response of X. sorbifolium to salt mist stress. These findings provide a theoretical foundation for clarifying the biological functions of XsAP2/ERF genes and identifying salt-tolerant genetic resources in X. sorbifolium.

     

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