Abstract:
Objective This study aimed to identify members of the MADS-MIKC gene family in sea buckthorn, and to analyze their physicochemical properties, evolutionary relationships, gene structures and expression patterns, thereby providing a molecular basis for breeding high-quality sea buckthorn germplasm resources and elucidating regulatory mechanisms underlying fruit development.
Method Using the laboratory's sea buckthorn genome data, bioinformatics methods were used to identify the members of the gene family, and combined with transcriptome sequencing and RT-qPCR to verify the expression patterns of the genes during the development of sea buckthorn pulp and seeds.
Result A total of 55 MADS-MIKC gene family members were identified in the sea buckthorn genome and divided into 11 subfamilies, all genes contained introns. Promoter analyses indicated that most genes harboured multiple cis-acting elements associated with responses to abscisic acid, auxin, and gibberellin. The MADS-MIKC gene family was unevenly distributed across 12 chromosomes, and segmental duplication appeared to be the primary driver of family expansion. Expression heatmap and RT-qPCR analysis showed that the gene family exhibited significant expression differences in different developmental stages and tissues: the SEP and SHP subfamilies were highly expressed in pulp and seeds, and RT-qPCR verified the reliability of the transcriptome data.
Conclusion The sea buckthorn MADS-MIKC gene family has tissue- and stage-specific regulatory roles in the process of fruit development, which provides a theoretical basis for analyzing the fruit ripening mechanism of sea buckthorn and molecular breeding.
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