杨树<i>PIF</i>基因家族成员表达模式研究

王丽娟; 任逸秋; 李煜; 张利; 徐向东; 卢孟柱

doi:10.13275/j.cnki.lykxyj.2018.02.003

杨树PIF基因家族成员表达模式研究

Analysis of Expression Pattern of PIF Family Members in Populus

摘要

摘要:
目的为探究光敏色素相互作用因子(PIF)基因在杨树生长发育中的功能。
方法利用生物信息学方法预测了杨树基因组中的PIF基因家族成员并对各成员的基因结构、保守基序进行了系统分析; 在此基础上, 采用实时荧光定量PCR技术检测了PIF成员在不同组织及低温、干旱和NaCl胁迫下的表达模式。
结果表明:杨树基因组中至少含有10个PtPIF成员, 这些成员在进化上相对保守, 均具有典型的bHLH结构域。基因表达分析显示:PtPIF基因在杨树不同组织及不同非生物胁迫条件下存在明显的表达差异。
结论杨树PtPIF基因家族包含10个成员参与不同的生物学过程, 研究结果将为深入解析杨树PtPIF基因的功能奠定了基础。

Abstract:
Objective To study the function of phytochrome interacting factor (PIF) in Populus.
Method The molecular characteristics analysis of PIF was conducted, including gene family members, gene structure and conserved motifs. The expression profiles of PIF family members in different tissues were tested under cold, drought and NaCl stresses with real-time quantitative PCR.
Result The results of bioinformatics analysis showed that there were at least ten PtPIF members in Populus and the PtPIF genes were conserved during evolution of species, all of them contained bHLH domain.
Conclusion The result of qRT-PCR revealed that PtPIF has distinct expression pattern across different tissues and under different abiotic stress conditions. Present study provides references for further decipher the function of PtPIF in Populus.

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