Abstract:
Objective To study the function of phytochrome interacting factor (PIF) in Populus.
Method The molecular characteristics analysis of PIF was conducted, including gene family members, gene structure and conserved motifs. The expression profiles of PIF family members in different tissues were tested under cold, drought and NaCl stresses with real-time quantitative PCR.
Result The results of bioinformatics analysis showed that there were at least ten PtPIF members in Populus and the PtPIF genes were conserved during evolution of species, all of them contained bHLH domain.
Conclusion The result of qRT-PCR revealed that PtPIF has distinct expression pattern across different tissues and under different abiotic stress conditions. Present study provides references for further decipher the function of PtPIF in Populus.