Abstract:
Objective Many genes coding for important regulatory factors in model plant appears amplified numbers in woody plants. It is worth to be further investigated that the Class A ARR gene family, as a kind of cytokinin responsive factor, reduces its number in Populus.
Method Based on the method of bioinformatics, the expression pattern of family members was analyzed by RNA-seq and semi-quantitative PCR, building PtRRI fused to GUS expression vector, Detecting the PtRRI expression in Populus alba×P. glandulosa clone after treated with 6-BA, GA3, ABA and IBA hormones and the GUS signals of PPtRRI::GUS transgenic plant in the process of rooting.
Result The GUS signal in PPtRRI::GUS transgenic plants indicated that the PtRRI was mainly expressed in roots, cambium, xylem and this gene may involve in the secondary growth and the expression pattern during adventitious root formation. In addition, the PtRRI transcription expression was induced by 6-BA, which was consistent with its expression under hormone regulation during adventitious root development.
Conclusion The results of this study suggest that PtRRs may involve in the process of secondary growth and development of Populus.