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不同油茶物种及品种果实中甘油三酯成分分析

Analysis of Triacylglycerols in Different Oil-tea Camellia Cones

    摘要
  • 摘要:
    目的 探明不同油茶物种及品种果实中甘油三酯的组成。
    方法 采用HPLC-MS、主成分分析方法对6个不同油茶物种和9个不同普通油茶品种果实中甘油三酯成分进行分析。
    结果 表明:共检测出24种甘油三酯, 主要为OOO(三油酸甘油三酯)、OOL(1, 2-二油酸-3-亚油酸甘油三酯)、OOP(1, 2-二油酸-3-棕榈酸甘油三酯)等不饱和甘油三酯, 其中, 不同油茶物种果实OOO、OOL含量较高的均为浙江红花油茶(21.18%、17.70%), OOP含量较高的为普通油茶(14.62%); 不同普通油茶品种果实OOO含量较高的为长林166号(45.83%), OOL含量较高的为长林18号(21.05%), OOP含量较高的为长林53号(16.90%), 含量均超过10.00%。不同油茶物种和品种果实甘油三酯的综合排名与其含油率排名均存在差异。
    结论 结合含油率与甘油三酯主成分分析结果得出, 不同油茶物种果实中, 浙江红花油茶表现较优, 不同普通油茶品种果实中, 长林21号表现较优。研究结果为油茶籽油指纹图谱构建、品质鉴定提供了依据。

     

    Abstract:
    Objective To analyze the content of triacylglycerols (TAGs) in different oil-tea camellia cones.
    Method The TAGs composition of six oil-tea camellia species and nine varieties of Camellia oleifera cones were analyzed by HPLC-MS and principal component analysis (PCA).
    Result Twenty-four kinds of TAGs were detected, mainly OOO (triolein), OOL (1, 2-dioleoyl-3-linoleoyl-rac-glycerol) and OOP (1, 2-dioleoyl-3-palmitoyl-rac-glycerol). Among oil-tea camellia species, the cones of C. chekiangoleosa had the highest contents of OOO and OOL (21.18%, 17.70%), the common C.oleifera had the highest OOP content (14.62%). Among the common C. oleifera species, Changlin-166 had higher OOO (45.83%, Changlin-18 had higher OOL (21.05%), and Changlin-53 had higher OOP (16.90%), all of them were over 10%. The comprehensive evaluation ranking of TAGs was inconsistent with the oil content in different oil-tea camellia cones.
    Conclusion From the analysis of oil content and PCA of TAGs, it is concluded that the nutritional value of C. chekiangoleosa is better among oil-tea camellia species and Changlin-21 is better among common varieties of C. oleifera. The results could provide references in the construction of fingerprint and quality identification of oil-tea camellia oil.

     

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