Abstract:
Objective To select suitable quantitative real-time PCR (qRT-PCR) reference genes for studying culm shape development of Dendrocalamus sinicus Chia et J. L. Sun.
Method PCR and qRT-PCR were used to analyze the mRNA expression stability of six candidate reference genes (EF-1α, GAPDH, Actin, TIP-41, Tubulin and PP2A) in a set of six bamboo shoot samples at three development stages of the straight-culmed and bending-culmed D. sinicus respectively. The software GeNorm and NomFinder were employed to evaluate the data for reference genes. Quantitative analysis of PTAL was used to test the effectiveness of the candidate reference genes.
Result The results showed that six candidate reference genes were of expected size and single peaking melting curve. Of them, Actin, EF-1α and GAPDH had the best stability and correlativity. Meanwhile, these three candidate reference genes had the highest efficiency based on quantitative analysis results of the PTAL gene during the culm development.
Conclusion The Actin, EF-1α and GAPDH were optional inference genes for normalizing purpose genes in studying culm shape development of D. sinicus. The study can provide references for gene expression analysis in the culm development of D. sinicus.