高级检索+

油茶不同品种抗炭疽病与相关酶活性关系的研究

Study on the Relationship Between Enzyme Activity and Disease Resistance of Different Camellia oleifera Varieties to Colletotrichum gloeosporioides Penz

  • 摘要:
    目的 探明6种供试油茶的抗炭疽病等级, 分析油茶过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)4种酶活性, 摸清油茶抗炭疽病与酶活性的相关程度。
    方法 采用活体针刺接种自然发病法评价抗炭疽病等级, 采用光度分析法测试了酶活性, 采用关联法分析抗炭疽病与相关酶活性的相关程度。
    结果 结果表明:感病指数最小为博白大果油茶26.9, 病斑长度(2.62±0.40) mm, 感病指数最大为普通油茶68.8, 病斑长度(8.01±2.03) mm。感病指数大小顺序依次为:普通油茶 > 岑软3号 > 岑软2号 > 陆川油茶 > 香花油茶 > 博白大果油茶; 感病后, 高抗的博白大果油茶、中抗的陆川油茶和香花油茶POD活性均较其他高感油茶品种明显升高, CAT也明显升高(除岑软2号油茶外), SOD和PPO则表现各异; 防御酶活性诱导增长率、酶AUEAPC综合增长率以高抗的博白大果油茶, 中抗的香花油茶和陆川油茶增长率高于高感油茶; 健康油茶酶活性与病斑大小、感病指数相关性弱或基本不相关, 感病后POD、CAT、PPO均与抗性呈正相关, 而SOD与抗性呈负相关, 相关性均在高度和中度以上。
    结论 博白大果油茶为高抗品种, 陆川及香花油茶为中抗品种, 岑软2号、岑软3号、普通油茶为高感品种, 且感病油茶酶活性与感病指数存在明显相关, 试验进一步明确了油茶抗炭疽病及其防御酶在抗性评价中的作用, 为早期鉴别油茶抗病性并筛选抗病油茶提供参考依据。

     

    Abstract:
    Objective To study 6 Camellia oleifera varieties' disease resistance, the peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and polyphenol oxidase (PPO) activity, the correlation of disease resistance and the enzyme activity.
    Method The living wounding inoculation method was used to evaluate disease resistance, the enzyme activities was tested by photometric analysis, the disease resistance and the relevance of the enzyme activity were studied by correlation analysis.
    Result The results show that the disease infected index of C. gigantocarpa was 26.9, disease length (2.62±0.40) mm, the disease infected index of Camellia oleifera was 68.8, disease length (8.01±2.03) mm, and the disease resistance was in the order of C. gigantocarpa > C. osmantha Ye CX > C. vietnamensis > C. oleifera 'Cenruan 2'> C. oleifera 'Cenruan 3' > Camellia oleifera. The SOD and PPD activities were different. The Defense enzymes induced growth rate, the enzyme AUEAPC comprehensive growth rate of high and medial resistant varieties was higher than that of the highly susceptible varieties. The lesion size, infectious index was uncorrelated with the healthy plants enzyme activity, but the POD, CAT, PPO was high positive correlated with the diseased plants enzyme activity, the SOD was high negative correlation with the diseased plants enzyme activity.
    Conclusion C. gigantocarpa belongs to high resistant variety; C. vietnamensis and C. osmantha the medial resistant varieties, Camellia oleifera, C. oleifera 'Cenruan 2', C. oleifera 'Cenruan 3' belong to highly susceptible varieties. A clear correlation is found between disease infected index and the enzyme activity. This conclusion further clarifies the disease resistance of C. oleifera and enzymes evaluation, could also be useful for the early identification of disease resistance of C. oleifera.

     

/

返回文章
返回