Abstract:
Objective To study 6 Camellia oleifera varieties' disease resistance, the peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and polyphenol oxidase (PPO) activity, the correlation of disease resistance and the enzyme activity.
Method The living wounding inoculation method was used to evaluate disease resistance, the enzyme activities was tested by photometric analysis, the disease resistance and the relevance of the enzyme activity were studied by correlation analysis.
Result The results show that the disease infected index of C. gigantocarpa was 26.9, disease length (2.62±0.40) mm, the disease infected index of Camellia oleifera was 68.8, disease length (8.01±2.03) mm, and the disease resistance was in the order of C. gigantocarpa > C. osmantha Ye CX > C. vietnamensis > C. oleifera 'Cenruan 2'> C. oleifera 'Cenruan 3' > Camellia oleifera. The SOD and PPD activities were different. The Defense enzymes induced growth rate, the enzyme AUEAPC comprehensive growth rate of high and medial resistant varieties was higher than that of the highly susceptible varieties. The lesion size, infectious index was uncorrelated with the healthy plants enzyme activity, but the POD, CAT, PPO was high positive correlated with the diseased plants enzyme activity, the SOD was high negative correlation with the diseased plants enzyme activity.
Conclusion C. gigantocarpa belongs to high resistant variety; C. vietnamensis and C. osmantha the medial resistant varieties, Camellia oleifera, C. oleifera 'Cenruan 2', C. oleifera 'Cenruan 3' belong to highly susceptible varieties. A clear correlation is found between disease infected index and the enzyme activity. This conclusion further clarifies the disease resistance of C. oleifera and enzymes evaluation, could also be useful for the early identification of disease resistance of C. oleifera.