9种瘿蚜的<i>Wolbachia</i>分子检测和系统发育分析

杨瑛; 吴海霞; 任维宾; 陈航; 杨子祥

doi:10.13275/j.cnki.lykxyj.2018.03.001

9种瘿蚜的Wolbachia分子检测和系统发育分析

Molecular Detection and Phylogenetic Analysis of Wolbachia in Nine Gall Aphid Species

摘要

摘要:
目的以五倍子蚜、杨树瘿蚜和黄连木瘿蚜等9种瘿蚜为实验材料，探讨瘿蚜Wolbachia与其它自由生活蚜虫携带的Wolbachia的株系差异及亲缘关系，明确五倍子蚜、杨树瘿蚜和黄连木瘿蚜等9种瘿蚜的Wolbachia感染情况、株系及系统发育关系。
方法从田间采集新鲜虫瘿，将蚜虫转移到离心管内，100%乙醇-20℃保存。采用Wolbachia的16S rRNA、wsp、ftsZ、groE和gltA基因引物，以瘿蚜的总DNA为模板进行扩增、测序和分析，联合NCBI中Wolbachia 16S rRNA相关基因序列进行系统发育分析。
结果在检测的9种瘿蚜中，有3种瘿蚜共3个种群感染有Wolbachia，其中滇叶瘿绵蚜昆明种群的Wolbachia属于B超群，肚倍蚜昆明种群和角倍蚜峨眉种群的Wolbachia可能属于新发现的O超群。
结论与自由生活的蚜虫相比，瘿蚜的Wolbachia感染率偏低，且株系间的差异较大。

Abstract:
Objective To determine the infection and phylogenetic relationships of Wolbachia in nine gall aphids hosted on Rhus spp., Populus yunnanensis and Pistacia chinensis.
Method Fresh aphid galls were collected in the field and transferred the aphids to an Eppendorf tube, and placed in 100% ethanol then deposited in -20℃. The genomic DNA was amplified and sequenced using the primers of 16S rRNA, wsp, ftsZ, groE and gltA genes. The phylogenetic relationships were analysis using Wolbachia 16S rRNA gene sequences and relevant gene sequences from NCBI.
Result Three species among the nine gall aphids species were infected with Wolbachia. The strain from Pemphigus yunnanensis of Kunming population belongs to B supergroup, and strain from Kaburagia rhusicola of Kunming population and Schlechtendalia chinensis of Emei population belong to O supergroup.
Conclusion Compared with the free-living aphids, Wolbachia infected in gall aphids have low infection rates and significant differences among strains.

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