Abstract:
Objective To analyze the phylogenetic position and genetic evolution of Pagiophloeus tsushimanus.
Method Four DNA sequence fragments: cytochrome oxidase subunitⅠ(COI) gene of mitochondrial DNA (mtDNA), V4 and V7 region of 18S ribosomal DNA (18S rDNA) and D2 region of 28S ribosomal DNA (28S rDNA) were amplified. Maximum likelihood (ML) and neighbor jointing (NJ) methods were used to build up phylogenetic trees of P. tsushimanus and related species in Curculionidae, so as to study the classification status of P. tsushimanus.
Result Analysis on COI and rDNA suggested that P. tsushimanus belongs to Hylobiini of Molytinae.
Conclusion According to the analysis, the results of 28S rDNA support the morphological classification of P. tsushimanus.