Abstract:
Objective To study the sequence characteristics of Phalaenopsis equestris.
Method BLASTP was used to search the MYB transcription factors of P. equestris (PeMYBs) in the database of P. equestris whole genome, and then the conserved MYB domain and structural characteristics of PeMYBs were predicted. The sequence alignment, phylogenetic analysis and expression profiles of PeMYBs were conducted by using ClustalW, MEGA and HemI 1.0 softwares.
Result 96 PeMYBs have conserved R2R3-MYB domains, 27 have R1-MYB domains, and 2 have R1R2R3-MYB domains. According to the classification criterion, the 96 R2R3-PeMYB members could be divided into 20 groups, and among them, some PeMYB genes were orthologs and PeMYB/AtMYB paralogs. They had different profiles in four organs, among them 39 PeMYBs were expressed in all the four organs, and 48 PeMYBs were specific expression or no expression in different organs.
Conclusion The results of this study could provide references for further functional analysis of MYB genes in P. equestris.