Abstract:
Objective The study aims at exploring and excavating the potential function of large genome fragment cloned from Populus euphratica, and finding out the potential gene clusters with breeding value.
Method Based on BIBAC library, the fragment 78A2D10 from the genome of P. euphratica, was inserted into the genome of Arabidopsis thaliana by the method of floral-dip. Resistance selection, molecular identification and phenotypic observation were applied to identify and analyze the transgenic plants.
Result Fifteen transgenic plants with specific phenotype were obtained in the research. Compared with the wild plants, the positive plants showed the characters such as inhibited growth of the stems, 3 times rosette leaf area, more leaves, crimping blade edge, delayed bolting with 13 weeks, height increasing by 32.0 cm, developing lateral stems and prolonged lifetime with 7 weeks.
Conclusion The fragment of 78A2D10 may prolong the vegetative growth and lifetime of plants. The fragment may be related to the vegetative growth of plants.
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