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5个观赏雷竹变型对低温胁迫的生理响应

Physiological Response of Five Varieties of Ornamental Phyllostachys praecox ‘Prevernalis’ to Low Temperature Stress

    摘要
  • 摘要:
    目的 比较不同程度低温胁迫对5个观赏雷竹变型生理指标的影响，研究不同雷竹变型的低温耐受生理学特性，为观赏雷竹引种和栽培提供技术依据。
    方法 将5个雷竹变型叶片于7个温度（0、−5、−10、−15、−20、−25、−30℃）下处理24 h，测定叶片相对电导率、抗氧化酶活性（超氧物歧化酶(SOD)、过氧化物酶（POD）、过氧化氢酶（CAT））以及可溶性蛋白、丙二醛（MDA）和脯氨酸含量。经Logistic方程拟合获得5个雷竹变型的半致死温度（LT50），通过主成分分析（PCA）和冗余分析（RDA），探究5个雷竹变型的生理指标与温度的相关性以及抗寒能力。
    结果 5个雷竹变型的相对电导率曲线均符合典型的“S”型曲线，LT50为弯秆雷竹（−18.73℃）< 细叶乌头雷竹（−17.09℃）< 黄条雷竹（−16.98℃）< 黄皮雷竹（−16.82℃）< 花秆雷竹（−14.57℃）。5个雷竹变型抗氧化酶活性及可溶性蛋白、MDA、脯氨酸含量均随温度的下降呈先上升后下降的趋势。可溶性蛋白和脯氨酸在第1主成分中载荷量较高，而SOD和POD活性在第2主成分中载荷较高。
    结论 5个雷竹变型耐寒性综合排名为：弯秆雷竹 > 细叶乌头雷竹 > 黄皮雷竹 > 黄条雷竹 > 花秆雷竹。渗透调节物质（可溶性蛋白和脯氨酸）和抗氧化酶（SOD 和POD）在雷竹耐寒过程中发挥主要作用。

     

    Abstract:
    Objective To observe the effects of cold stress on the physiology indicators of Phyllostachys praecox ‘Prevernalis’, in order to study the cold tolerance mechanism of five varieties of ornamental Ph. praecox ‘Prevernalis’ to cold stress.
    Method The relative conductivity, antioxidant enzyme activity (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)), soluble protein content, malondialdehyde content and proline content in leaves of Ph. praecox ‘Prevernalis’ treated at different temperatures (0, −5, −10, −15, −20, −25, and −30℃) for 24 hours were measured. The semi-lethal temperature (LT50) of the five varieties was obtained by fitting the Logistic equation, and the correlation between physiological parameters and temperature as well as the cold resistance of the five varieties were investigated by principal component analysis (PCA) and redundancy analysis (RDA).
    ResultsAll the relative conductivity curves of the five varieties were similar to S curve. The LT50 of the five varieties showed an increasing order of Ph. praecox cv. Linanensis (−18.73℃) < Ph. praecox ‘Xiyewutouleizhu’ (−17.09℃) < Ph. praecox f. Notata (−16.98℃) < Ph. praecox f. Chrysoderma (−16.82℃) < Ph. praecox f. Viridisulcata (−14.57℃). The antioxidant enzyme activity, soluble protein content, MDA content and proline content of the five varieties increased and then decreased with the decrease of temperature. The component matrix of soluble protein content and proline content was higher in the principal component 1. The activity of SOD and POD was higher in the principal component 2.
    Conclusion The cold tolerance of the five varieties showed a decreasing order of Ph. praecox cv. Linanensis > Ph. praecox ‘Xiyewutouleizhu’ > Ph. praecox f. Chrysoderma > Ph. praecox f. Notata > Ph. praecox f. Viridisulcata. The osmotic regulatory substances (soluble protein and proline) and antioxidant enzyme (SOD and POD) play major roles in the cold tolerance of Ph. praecox ‘Prevernalis’.

     

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