Abstract:
Objective The role of MYB transcription factor family in pigment formation of yellow Paeonia delavayi flowers was analyzed to provide candidate genes for color improvement and molecular breeding of tree peony.
Method In this study, members of MYB gene family of P. delavayi were identified and classified based on the transcriptome data of petals. One member of R2R3-MYBs, PdMYB2, was selected as a candidate gene according to phylogenetic tree, homologous sequence alignment and gene differential expression analysis. The function of PdMYB2 was analyzed by subcellular localization, qRT-PCR, transient overexpression, transgenic tobacco and LC-MS/MS.
Result A total of 64 MYB genes were identified, including 25 R2R3-MYBs, one R1R2R3-MYBs, two 4R-MYBs and 36 members of MYB-related. The PdMYB2 was found to express in the nucleus and cytoplasm. Overexpression of PdMYB2 in tobacco increased anthocyanin accumulation and up-regulated the expression of anthocyanin-related structural genes NtCHS and NtCHI in petals and leaves.
Conclusion PdMYB2 plays a positive regulatory role in the flavonoid biosynthesis pathway, which can promote the accumulation of anthocyanins and increase the expression of structural genes in the flavonoid pathway.
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