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不同浓度石墨烯对杉木幼苗生长、根系形态及15N吸收利用的影响

Effect of Different Concentrations of Graphene on the Growth, Root Morphology and 15N Uptake and Utilization of Cunninghamia lanceolata Seedlings

  • 摘要:
    目的 探究石墨烯对杉木幼苗生长、根系形态及N素吸收利用的影响,为杉木高效培育奠定理论依据。
    方法 以1年生杉木优良无性系“洋061”为试验材料,采用盆栽试验及15N示踪技术,测定不同浓度石墨烯(0、20、25、30 mg·L−1)处理的杉木幼苗生长、根系形态指标及15N分配率和利用率。
    结果 在杉木幼苗的生长和根系形态指标中,除茎干质量和根体积外,其余指标均表现为25 mg·L−1石墨烯浓度的处理最大,但各处理间差异不显著;杉木全株15N利用率表现为 25 mg·L−1>20 mg·L−1>30 mg·L−1>CK1,15N分配在杉木幼苗各器官表现为叶>茎>根;根15N分配率、全株15N利用率与根系形态的相关性显示,除根长、根体积、根15N分配率与根直径呈负相关外,其他各指标间均呈极显著正相关。
    结论 一定浓度的石墨烯有利杉木幼苗生长,并能促进N素吸收利用。

     

    Abstract:
    Objective To provide theoretical basis for the efficient cultivation of C. lanceolate, the effects of graphene on the growth, root morphology and nitrogen uptake and utilization of Cunninghamia lanceolata were investigated in this study.
    Method A pot experiment with four concentrations of graphene (0, 20, 25, 30 mg·L−1) was conducted in this study by using the superior clone of C. lanceolata seedlings ‘Yang 061’ to investigate the growth, root morphology, 15N distribution and utilization rate of C. lanceolata seedlings under various graphene treatments.
    ResultsThe indexes of growth and root morphologies were maximum at 25 mg·L−1 graphene concentration except for stem weight and root volume, but no significant difference were observed among different treatments. The 15N utilization rate of seedlings was 25 mg·L−1>20 mg·L−1>30 mg·L−1>CK1, and the distribution of 15N in different organs of C. lanceolata seedlings was leaf > stems > roots. Correlation analysis results showed that a significantly positive correlation between roots 15N partition rate and seedlings 15N utilization rate with different growth indexes were observed except for root length, root volume, root 15N partition rate and root diameter.
    Conclusion A certain concentration of graphene is beneficial to the growth of C. lanceolata seedlings and can promote the absorption and utilization of N.

     

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