Abstract: Seven EST-CAPS markerswere identified from 54 candidate ESTs for genetic mapp ingwith an F1 population of Eucalyptus urophylla ×E. tereticornis, including three specific in female parent (one being aberrant from normal segregation among the sibs) , two specific in male parent, and two shared by both parents. Four EST2CAPSmarkers were integrated to independent linkage group s on the RAPD linkage map of E. urophylla constructed p reviously, which contributed to some degrees to the extension of linkage group length. Also, four EST2CAPSs weremapped to different linkage group s on the RAPD linkage map of E. tereticornis, which gave rise to the increase oflinkage group length. In addition, the aberrantmarkerwas found to be unlinked to any linkage group in E. urophylla. This study demonstrated the usefulness of EST-CAPSmarkers in genetic mapp ing in Euca lyptus.