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松材线虫和拟松材线虫的杂交遗传差异研究*

Hybridization between Bursaphelenchus xylophilus and B.mucronatus

  • 摘要: 运用杂交方法对来自南京、四川、日本、法国和加拿大等地的松材线虫和拟松材线虫共10个株系作遗传分析,结果表明中国的松材线虫和拟松材线虫能杂交成功,但后代数量少且大多不孕,两者间遗传差异较大,这两种线虫各株系间无明显差异.当中国线虫分别与日本、法国、加拿大株系杂交时,无论种间或种内均有差异,前者差异更显着.种间杂交中多数后代雌虫较难与父本回交产生子代幼虫.种间杂交在产生正常后代的同时也产生10%~60%的畸形后代.雄虫畸形主要表现在无交合刺或交合刺不发育,雌虫和幼虫的畸形则是身体的缢缩、弯折等.

     

    Abstract: The genetic analysis was conducted by crossing experiments to use 5 strains of B.xylophilus from China,Japan,Canada and 5 strains of B.mucronatus from China,Japan,France.The results indicated that the two species of China might cross successfully,but the number of hybrids were limited and hardly fertile,so the two species were different in genetics.There was no difference in the strains of conspecies from China.When the two Chinese species were crossed with the strains of Japan,France,and Canada respectively,there was ovious difference in interspecific crossing and some difference in intraspecific crossing.The F1 generation females of interspecific hybridization had mucronate tails similar to B.mucronatus in reciprocal crossing.Back crossing of F1 hybrid females to their parent males were more difficult to produce progeny.The rate of deformations in hybrid adults ranged 10%~60%.Major deformations were no spicules in males and crooking of bodies in females and larvae.

     

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