Abstract: Objective To analyze the sequence features and gene expression rules in different tissues and periods of SPL transcription factor from Betula platyphylla Suk. Method Based on 45 transcriptome databases of Birch, 12 BpSPL genes were obtained and named as BpSPL1-BpSPL12, of which 11 genes were analyzed. Result Bioinformatics analysis showed that all the 11 BpSPLs contained a highly conserved SBP domain, their lengths exhibited a high diversity and owned 210 numbers of exon in genetic sequences. Phylogenetic analysis showed that the 11 Birch SPL proteins belonged to six major categories. The qRT-PCR results suggested that the expression levels of the 11 BpSPL genes changed significantly in different periods and different tissues (leaves, terminal buds, stems, male inflorescences). In terminal buds, most BpSPL genes were up-regulated on July 5 and August 20 to September 20. In male inflorescences, with the exception of BpSPL1 which was down-regulated or unchanged, all the other BpSPL genes exhibited a gradual increasing pattern from June to September (the growth stage). Conclusion This study suggested that these BpSPL genes may be involved in the growth and development of birch terminal buds and male inflorescences.