Abstract: This is an introduction of the research method on the nutrhion of trace elements and the effect on the growth of trees mainly for sand culture, including:the requirements of laboratory and green house;compounding and purification of nutritive fluids;the selection of experimental materials container for seedling,sand and water; the washing ofthe experiemental utensils; fertilization,sowing,watering and other managements of thegreen house; and the observation,recording; sample collecting and treatment and nutrientanalysis of the tested seedlings.