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无花果组织培养再生系统的研究

Tissue Culture and Regenerative System of Ficus carica

  • 摘要: 以无花果展叶腋芽、休眠腋芽、室外嫩茎和水培芽为外植体,通过初始培养获得无菌苗后,选取无菌苗的不同部位作外植体,如茎段、叶片、愈伤组织等,经过诱导培养、分化培养及生根培养,形成再生植株。

     

    Abstract: The methods of building the regenerative system of Ficus carica were studied, which would supply basic means and theoretical basis for its improvement and breeding and mechanism of inhibitory effect of NaCl. In this paper, shoot tip, axillary bud and dormant axillary bud were used as initial explant. After sterile shoots were obtained, different explants from them were cultured and regenerated into plantlets. Various media and hormones, hormone concentrations tested in induction. The best medium and hormone combination were suggested, and some problems of survival rate were also explored in transplanting plantlet experiment.

     

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