Abstract: Objective The purpose of this study is to obtain the bacterial strains with high antagonism against Colletotrichum gloeosporioides, Fusarium oxysporum and F. solani, the fungal pathogens of orchid. Method In this study,the method of dual culture on plate was used to screen the bacterial strains. Result It was found that there were 5 strains which could inhibit all the three pathogens and the strain GT312 had the highest antagonistic activity. The results of phylogenetic analysis of 16S rRNA gene sequence revealed that GT312 had 99.93% similarity with Bacillus amyloliquefaciens type strain FZB42T. Conclusion Based on the morphologic and physiological characteristics, the strain GT312 was identified as B. amyloliquefaciens. It could be used as a potential bio-control agent of orchid diseases.