Abstract: In this study,an efficient regeneration system of Broussonetia papyrifera was established.Using leaf disks and petiole fragments as explants,MS media supplemented with different concentrations of BA and NAA were investigated for shoot induction.Then regenerated shoots were transferred to MS medium containing 0～0.6 mg·L-1 NAA to induce roots.A BA/NAA combination of 2.5/0.1(mg·L-1) produced the highest percentage of regeneration with petiole explants and of 1.5/ 0.05(mg·L-1) with leaf explants.The optimal medium for rooting was MS medium supplemented with 0.05 mg·L-1 NAA.