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马尾松种子园无性系花粉生活力研究

Study on pollen viability of Clones from a Masson Pine Seed Orchard

  • 摘要: 借助7种培养液对采自马尾松无性系种子园27个无性系花粉生活力进行了测定.结果表明:不同无性系、不同培养基、无性系×培养基对花粉萌发率的作用均达到极显着水平;无性系间花粉粒大小差异极显着,并与发芽率间存在显着相关;通过主分量分析将不同无性系花粉按生活力高低分为三类:高生活力花粉、中生活力花粉、低生活力花粉.在使用的7种培养基中以5%蔗糖+50ppm或30ppm硼酸的组合培养效果最好.同时本文还就花粉生活力相关的问题进行了讨论.

     

    Abstract: Study on the pollen germination rate(PGR)of 27 clones from a clonal seed or chard of Pinus massoniana was reported in this paper. Seven solution media were used for the culture to evaluate the effects of different clones(A),incubating media(B)and their interaction (A×B) on pollen viability.In addition,the correlation between pollen size and PGR was analyzed.The results are as follows:(1)the influences of A,B and A×B on PGR were all at the level of extreme significance(P0.01);(2)there was isgnificant correlation between the pollen size and its germination rate,but no enough evidence has been found to regard pollen size as an equivalent parameter of PGR;(3)the pollens studied could be divided into three types batsed on their PGR through robust princiole component analysis;(4)the solutions containing 5% glucose and 30~50 ppm boric acid allowed the pollens to germinate best. Some problems on pollen viability test were also discussed with some suggestions.

     

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