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外生菌根真菌彩色豆马勃优良菌株营养源的研究*

Nurtitional Source Study for Super-srtain of Ectomycorrhizal Fungus,Pisolithus tinctorius

  • 摘要: 通过P.t.优良菌株9109在不同培养基上生长速度、培养特性及其生长规律的研究,筛选出生长最佳培养基作为基础培养基,进行C、N营养研究,试验结果表明:P.t.9109不仅能利用单糖、低聚糖和无机N等速效C、N源,而且能利用多糖、多元醇和有机N等长效C、N源。其菌丝生长最适C源是玉米粉和甘露糖;最适N源是蛋白胨和氯化铵。在无机N利用中,对硝态N和铵态N的利用无显着差异。应用L16(45)正交设计确定了P.t.9109菌丝生长的最佳营养配方,菌丝增殖高达860倍,不但提高菌丝生物产量,而且节省了原料,降低了成本,为改进P.t.菌剂生产配方和研制新型菌剂提供了最佳营养条件。

     

    Abstract: Through the study on growth rate,culture characteristics and growth rules of Pisolithus tinctorius on different medium,the optimal medium was selected as the basic medium for its carbon and nitrigen sources study. The results show that Pt has a wide range of nutritional sources for metabolization,not only can it use monosaccharides,disaccharides,and inorganic nitrogen, but also it can use polysaccharides, polyol and organic nitrogen. Corn meal and mannitol are its optimal carbon sources, peptone and NH4Cl the optimal nitrogen sources for its mycelial growth. No obvious difference was noted when amine and nitrate forms of inorganic nitrogen were utilized by the fungus. And the optimum formulae of nutrition for its mycelial growth are obtained through orthogonal experiment L16(45).Through using the formulae,the biomass of the fungal mycelium can astonishingly proliferate 860 times,which surpass that of the former report. Therefore,the research provided the theory base to improve the formula and the technology for producing the ectomycorrhizal inocula of the fungus.

     

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