两种省藤组培成苗芽的选择

Selection of in vitro Buds of Two Rattan Species for Elongation and Rooting

摘要: 对单叶省藤、短叶省藤组培快繁成苗芽的选择研究试验结果表明:生长健壮、形态正常的不同高度未展叶单芽组,在成苗培养过程中,高生长、生根率、生根条数和生根长度存在差异。高度在2.1～4.0cm的未展叶单芽为伸长培养和生根诱导培养的最佳高度组,生根诱导培养3个月后,芽苗生长可达到组培苗移植规格(苗高、根长4.0cm)。研究试验结果还显示,2种省藤组培成苗培养过程,伸长及生根诱导培养可浓缩为一步,直接从增殖培养材料中选择2.1～4.0cm的单芽进入生根诱导培养,一步成苗。

Abstract: This paper deals with the selection of in vitro buds of two rattan species for elongation and rooting.The results show that in vitro buds with different heights are significantly different in elongation and rooting.In vitro buds with the height of 2.1～4.0 cm are appropriate material for elongation and those with a height2.1 cm are good for rooting.Buds with a height2.1 cm can be selected for rooting without elongation culture.