Abstract: Giemsa C-band ing and FISH analysis of the Ph. pubescens var. heterocycla chromosomes from root tipswere carried ou t in this research. The resu lts showed that the C-band ing karyotypewou ld be 2n= 48= 4CI+ 2CI+ T+ 2CT+ + 10CT+ + 4I+ 2I+ + 6T+ + 6C+ 8T + 2IT + 2I+ T. The specific C-band ing points on each chromosomecou ld be distinguished and the d ifferences ofC-band ing intensity a lso could be recognized. 45S rDNA was used asprobe for FISH analysis, and one couple of hybridization signals was d isp layed on the chromosome centrimere. Inthis research we have a conclusion that chromosomes ofPh. pubescens var. heterocycla can be easily distinguishedand identified by Giemsa C-band ing and FISH approaches.