Abstract: The effects of sucrose concentration, pH value and storage conditions on germination and pollen tube growth of in vitro cultured spruce were studied, and the ingredients of culture medium were optimized. The results showed that the effect of sucrose concentration and pH value on spruce pollen’s germination was significant, and also a clear threshold effect existed. Low or high concentration of sucrose was not conducive to the pollen’s germination, it was found that 10% of which was more suitable to the in vitro culture of spruce pollen; different spruce species required different concentrations of sucrose medium, 7.5% of sucrose concentration medium was better to pollen tube germination of Picea asperata, but 10% of which was the best medium to that of Picea abies. Both pH 4.5 and 6.5 had restrained action on pollen germinating rate of spruce, and the growth of pollen tubes. Under dark culture conditions, the P. abies began to germinate in the 6 h, but the P. asperata germinated in 8 h, the P. abies was 2 hours in advance and in the mass germinating power was higher than that of P. asperata; the growth process of P. abies was 20-22 h, and that of P. asperata was about 16 h; The pollen tubes of P. abies was over 10 μm longer in length than that of P. asperata. There were significant differences of germinating power and length of pollen tubes between genotypes of two different individuals of both spruces. The storage methods had great influence on the viability of pollen. In the process of pollen preservation, temperature was the key factor, cryopreservation was better than cold storage and room temperature preservation was unfavorable.