Abstract: The explants of Cymbidium sinensis stems and spores were used to study Cymbidium sinensis tissue culture essentials. It dealed with the selection of explant, induction of proliferation, subculture, rooting and outplanting of Cymbidium sinensis. The results showed that the best medium to induce proliferation was MS+6-BA 4.0 mg·L-1+NAA 1.0 mg·L-1+agar 8 g·L-1+sugar 30 g·L-1, by wielding orthogonal test. While rooting medium was half MS and 3.0 mg·L-1of NAA. At the same time,we obtained by analyzing and contrasting the standard spore to bring about rooting, which offered basis of Cymbidium sinensis, such as enlarging produce, increasing living rate and improving economy benefits correspondingly.