Abstract: For p robing into the effects of different Rhizobia inoculated on Acacia crassica rpa tissue－cultured p lantlets,different Rhizobia were inoculated on Acacia crassicarpa tissue－cultured p lantlets. The results showed that the effectsof different Rhizobia on the p lantlet’s height growth, biomass, chlorophyll contents and nodulat rate were markedness. There were different appetency between different Rhizobia and host species. The strains HO6 and HA4 wereselected as superior strains based the mensuration results of the p lantlets.