Abstract: The shoots of Populus cathayana Rehd. var. Qinghai were used as explants for tissue culture to study the effects of different hormone concentrations on the plant's regeneration system and a high-efficient tissue culture regeneration system of P. cathayana Rehd. var. Qinghai was established. It was found that P. cathayana Rehd. var.Qinghai was very sensitive to hormone concentration. The concentration of hormone had great impact on their growth in various stages. The best culture medium for induction and differentiation of P. cathayana Rehd.var. Qinghai was 1/2MS+6-BA 0.5 mg·L-1+NAA 0.05 mg·L-1, on which the differentiation rate was 82.0%; The best culture medium for bud growth was MS+NAA 0.005 mg·L-1 ;The best culture medium for proliferation was MS+6-BA 0.1 mg·L-1+NAA 0.3 mg·L-1; The best culture medium for rooting was 1／2MS+IBA0.3 mg·L-1.