优良保健树大叶冬青组培扩繁的研究

Techniques of Tissue Culture and in vitro Clonal Propagation of Ilex latifolia

摘要: 以大叶冬青具节茎段芽体增殖产生试管苗,适宜培养基为MS+1.0μmolNAA+8.8μmolBA或MS+1.0μmolNAA+9.1μmolZT;幼叶外植体诱导的愈伤组织块转培于WPM+5.0μmolTDZ+3.0μmolIAA培养基上,能产生较多不定芽苗(每一愈伤组织块平均产生11.2株);BA对大叶冬青外植体不定芽的处理能产生较好的增殖系数与高生长;试管苗适宜生根处理为1/4MS+1.0μmolNAA(或+1.0μmolIBA);室外移栽成活率达90%。

Abstract: The optimum medium for in vitro growth and multiplication of Ilex latifolia from nodal segments was MS+1.0 μmol NAA+8.8 μmol BA or MS+1.0 μmol NAA+9.1 μmol ZT. When calli resulted from cultured young leaves were transferred to WPM supplemented with 5.0 μmol TDZ and 3.0 μmol IAA,the most of adventitious shoots (11.2 per callus) formed. And BA was suitable for the multiplication and height growth of adventitious shoots. 1/4 MS+1.0 μmol NAA(or+1.0 μmol IBA) optimized the rooting of shoots. And the plantlets got acclimatized well, with the survival up to 90%.