Abstract: The genome of Buzura suppressaria nucleopolyhedrovirus isolate Guangxi was sequenced by Sanger's method. The size of the genome is 121 268 bp with 36.76% G+C content. It contains 131 hypothetical open reading frames (ORFs) that potentially encode at least 50 amino acids respectively. 87 ORFs were annotated by Blast. 174 SSR sites and one homologous repeated sequence (HRS) were found in the genome. The SSR sites contained 6 types of repeats, and most of them were AT-rich sequences. HRS contained 12 repeats at 5' end and 7 repeats at 3' end. The repeat was 59 bp or truncated. Between 5' end and 3' end rapeats, there was a 236 bp sequence with no obvious features. Compared with Wuhan strain, 4 genes among 37 core genes were different in protein length and 6 non-core genes were found only in one strain, and another 13 genes were different in protein length.