Abstract: Euonymus evergreen hybrid(1#, 2#, 3#) and their parents (Euonymus japonicus‘Zhuzi’, E. kiautschovicus) were studied to test whether their cold resistance were stronger than their parents in the winter. The result showed The semi-lethal temperatures (LT50) were assessed separately by electrolyte leakage, the result showed that the LT50 of 1#, 2#, 3#, E. japonicus‘Zhuzi’ and E. kiautschovicus were 36℃, 32.1℃, -28.5℃, -26.9℃, and -28℃ respectively. The activities of superoxide dismutase (SOD), peroxidase (POD) in 3# were higher than that of the others, but there was no significant difference among the other varieties. The contents of proline in leaves of E. japonicus‘Zhuzi’ were higher than that of Euonymus evergreen hybrids and E. kiautschovicus. The present study indicates that the hybrid evergreen euonymus and their parents had different mechanisms of cold-resistance.