Abstract: In this study,the wood-rotting fungi were chosen to evaluate their relation with Bursaphelenchus xylophilus(PWN),the ability to decompose the wood specimens of Pinus massoniana and the stumps of dead pine trees caused by PWN.the results showed that all the wood-rotting fungi tested had certain nematicidal effects on PWN except Schizophyllum commune.Among the wood-rotting fungi,PWN could not survive on the strains of W10,W11(Fomitopsis pinicola),5452,6600(Laetiporus sulphureus),6221(Pleurotus ostreatus),6320(Tremellodon gelatinosum),6284(Poria cocos),6501(Ganoderma lucidum) absolutely.The results of the experiment indicated that the strains of W10,W11(Fomitopsis pinicola),6600(Laetiporus sulphureus),6923(Coriolus versicolor) and 6284(Poria cocos) had higher ability to decompose Pinus massoniana specimens.After inoculating the strains of 6600,6923,W11,6284,6221 to treat stumps for 70 d.The strains of 6600 and 6923 showed higher ability to decompose the stumps.The strains could be used to treat stumps of dead wood caused by PWN in the future.