Abstract: The biomass and nutrient contents of Eucalyptus urophylla cl. MLA plantation with early-stage fertilizer trial established at Kaiping, Guangdong, were measured and analyzed at 6 year old. The results showed that fertilizer applied at early-stage promoted the growth and increasing of biomass of E.urophylla cl. MLA plantation. The treatment with the best increment at 6 year old had the biggest biomass with the biomass on ground of 65.95 t·hm-2 and stem biomass of 54.94 t·hm-2, and 1.65 times and 1.67 times that of the treatment with the biggest fertilizer quantity respectively. The single biomass and stand biomass of E. urophylla cl. MLA plantation might be forecasted by the regression equation established through height, DBH and biomass. N, P and K in foliage had bigger nutrient concentrations. Fertilizers applied had promoted to absorb and accumulate to nutrient elements for E. urophylla clone.