Abstract: The anatomical study on the rooting stems of Eucalyptus ABL 12 W5,E.leizhou No, 18051 and E. urophylla indicated that there were not any latent root primodia inthe tissues of Eucalyptus cuttings. To induce rooting,various technical methods should beused.The root primodia could be induced to emerge from vascular cambia,phloem parenchyma cells, phloem rays, pith rays,callus,and the complex tissues including vascular cambiaphloem rays and phloem parenchyma cells. The induced root primodia could be differentiatedinto adventitious roots under appropriate environmental conditions.