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两种蛀干昆虫消化酶组成和活性比较

Comparison of Components and Activity of DigestiveEnzymes between Two Xylophagous Insects

  • 摘要: 研究了松墨天牛幼虫、纵坑切梢小蠹幼虫和成虫消化酶的主要种类和活性。分析结果表明, 松墨天牛幼虫有10种消化酶, 纵坑切梢小蠹幼虫和成虫有8种消化酶。松墨天牛幼虫的消化酶活性大小排列顺序为: 果胶酶> 淀粉酶> Cx酶> 半纤维素酶> 转化酶> β-1, 4??葡萄糖苷酶> C1酶> 脂肪酶> 类胰蛋白酶> 胃蛋白酶; 小蠹幼虫和成虫不含Cx酶和β-11, 4??葡萄糖苷酶, 活性顺序均为: 果胶酶> 淀粉酶> 半纤维素酶> 转化酶> C1酶> 类胰蛋白酶>脂肪酶> 胃蛋白酶。在55 ℃ 、pH 值8. 6时, 果胶酶活性最高。

     

    Abstract: The components and act iv ity of digestive enzymes ex tracted from larvae o fM onochamus alternatus, larvaeand adults o fTomicus p inip erda w ere ana lyzed in this paper. The results revealed tha,t there w ere 10 digestive en-zymes in Monochamus alternatus and 8 in Tom icus p inip erda. The activ ity order o f enzymes o f larvae ofMonochamusalternatus arranged as pectinase> amy lase> endo-β-1, 4-g lucanase ( Cx-ase) > hem icellu lase> invertase> -1, 4-glucosidase> exo-β-1, 4-g lucanase ( C1-ase) > lipase> trypsinlike enzym e> pepsin; and for larvae and adu lts ofTomicus p in ip erda, the activity order of enzymed arranged as pectinase> amy lase> hem ice llulase> invertase> exo-1, 4-glucanase( C1-ase) > trypsin like enzyme> lipase> pepsin. There w ere no Cx-ase and -1, 4-g lucosidase forboth larvae and adu lts o fTom icus p iniperda. The resu lt pect inase had the h ighest act iv ity under 55℃ and pH= 8. 6.

     

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