Abstract: Paulownia fortunei superior trees "Baiyou 2" were used as trial materials to explore the rejuvenation technology of P. fortunei superior tree by tissue culture and in vitro grafting. The results are as follows: (1) The sprouting of explant is the most suitable scion; (2) The Paulownia variety "Jianshi 3" is a good rootstock; (3) Split grafting is a suitable; (4) 1/2 MS+6-BA 4.0 mg·L-1+NAA 0.3 mg·L-1 is the most appropriate medium for in vitro grafting, 1/2 MS+6-BA 6.0 mg·L-1+NAA 0.3 mg·L-1+Sucrose 20 g·L-1 is the most appropriate for multiplication and 1/2 MS+NAA 0.1 mg·L-1 is the most appropriate for rooting. The old Paulownia superior tree material was rejuvenated successfully and the rapid propagation was realized.