Abstract: The cluster analysismethod of karyotype resemblance2near coefficient was used to study the relationshipand evolution of seven species in the genus Kerria. The results showed that K. ru ralis and K. yunnanensis had theclosest relationship with the nearest homology in karyotype. The value ofλ between K. sindica and K. lacca was0. 953 3 and the two species form a sister group, indicating a close relationship. K. pusana and K. nepalensis alsoformed a sister branch with the value ofλ 0. 944 1. The karyotype of K. chinensis ismuch different from the othersix species and formed an isolated branch. On the evolution trend of karyotype, K. pusana and K. nepalensis werethe earliest diverging members and they were the p rimitive species in the group as the value ofλ between the twospecies and the otherswas no more than 0. 762 6. K. sind ica and K. lacca also belonged to p rior species as theydiverged earlier than K. chinensis. On the contrary, K. ru ralis and K. yunnanensis had the highest identity inkaryotype and nearest distance in evolution, which showed they were the latest species of the seven-group. K.chinensis belonged to more advanced category. The evolution trend of chromosomes was app roximately along thedirection from asymmetry to symmetry.